Mesothelin Antibody

• Catalog Number: orb757396
• Category: Antibodies
• Description: Anti-MSLN antibody(DM73), Rabbit mAb
• Target: Mesothelin
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Purification: Purified from cell culture supernatant by affinity chromatography
• UniProt ID: Q13421
• Tested applications: ELISA, FC, WB
• Dilution range: ELISA 1/5000-10000|Flow Cyt 1/100
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Antibody Type: Recombinant Antibody
• Clone Number: DM73
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MSLN, Mesothelin, MPF
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human MSLN protein, mFc-His tagged protein orb689392 can bind Rabbit anti-MSLN monoclonal antibody (clone: DM73) in a linear range of 1-100 ng/ml.

Expi 293 cell line transfected with irrelevant protein (A) and human mesothelin (B) were surface stained with Rabbit anti-MSLN monoclonal antibody 1 μg/ml (clone: DM73) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

Flow cytometry data of serially titrated Rabbit anti-MSLN monoclonal antibody (clone: DM73) on Hela cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Affinity ranking of different Rabbit anti-MSLN mAb clones by titration of different concentration onto Hela cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Flow cytometry analysis of antigen binding of rabbit anti-human Mesothelin mAb (orb757396). (A) orb757396 does not bind to 293T cells that do not express Mesothelin. (B) A clear peak shift of orb757396 was seen compared to the control when incubated with Mesothelin-expressing Hela cells, indicating strong binding of orb757396 to Mesothelin. Antibodies were incubated at 2 μg/mL.

Anti-MSLN antibody (SKU# orb757396) at 1/1000 dilution.Lane: HeLa (human cervical adenocarcinoma epithelial cell), whole cell lysate.Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution.Predicted band size: 69 kDa.Observed band size: 50 kDa