Mesothelin antibody

SKU: orb2276572

Description

Mesothelin antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

• Tested Applications: IHC
• Dilution range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Tonsil: A fraction of squamous epithelial cells of the tonsil crypts should show strong mesothelin immunostaining. Fallopian tube: An at least moderate mesothelin positivity should be seen at the apical membrane of epithelial cells. Negative Control: Tonsil: Squamous epithelial cells of the tonsil surface and all lymphocytes must not show mesothelin immunostaining. Cellular Localization: Cell Surface and Secreted Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone No.: Biorbyt-235M
• Immunogen: Recombinant fragment (around aa 273-407) of human Mesothelin (MSLN) protein (exact sequence is proprietary)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

CAK1; Megakaryocyte potentiating factor; Mesothelin; MSLN; SMR; SMRP

Breast Adenoid cystic breast cancer with strong membranous mesothelin immunostaining in a subset of cells.

Ovary Strong predominantly membranous mesothelin staining in an endometroid ovarian cancer.

Stomach Strong predominantly cytoplasmic mesothelin immunostaining in a stomach cancer with diffuse growth pattern.

Quick Database Links

UniProt

Q13421