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MEK1/2 Rabbit mAb
Description
Research Area
Images & Validation
−| Tested Applications | ICC, IF, IHC-Fr, IHC-P, IP, WB |
|---|---|
| Dilution Range | WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone No. | R08-6A7 |
| Immunogen | Recombinant protein of human MEK1/2 |
| Target | MAP2K1/MAP2K2 |
| Molecular Weight | Calculated MW: 43,44 kDa; Observed MW: 43,44 kDa |
| Purification | Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% rAlbumin. |
| Concentration | 0.3 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Western blot analysis of MEK1/2 in K562, C6, 3T3, Hela lysates using MEK1/2 antibody.

Immunocytochemistry analysis of MEK1/2 (green) in Hela using MEK1/2 antibody, and DAPI (blue).

Immunohistochemistry analysis of paraffin-embedded Human colon cancer using MEK1/2 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Protocol Information
MEK1/2 Rabbit mAb (orb1563050)
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