mCherry Antibody

• Catalog Number: orb1338813
• Category: Antibodies
• Description: Mouse monoclonal antibody to Carrier-free (BSA/glycerol-free) mCherry
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: OTI10G6 (formerly 10G6)
• Tested applications: WB
• Isotype: IgG1
• Immunogen: This antibody is raised against a recombinant protein of mCherry expressed in HEK293 cells.
• Dilution range: WB: 1:2000
• Conjugation: Unconjugated
• MW: 26.7 kDa
• Target: mCherry
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Note: For research use only
• Application notes: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
• Expiration Date: 12 months from date of receipt.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY mCherry (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-mCherry (1:200