mCherry antibodyCustom conjugation service available

Catalog Number: orb66657

References (2)

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Catalog Numberorb66657
Species/HostMouse
ClonalityMonoclonal
Tested applicationsELISA, IHC-P, WB
ReactivityHuman, Rat
Clone ID5A12A7H10
IsotypeMouse IgG1
Immunogen

Human mCherry recombinant protein (orb178365) was used as immunogen.

Concentration

- 100 μg (in 200 μl): 0.5 mg/ml
- 200 μg (in 400 μl): 0.5 mg/ml

Dilution rangeWB: 1 μg/ml
Form/Appearance10 mM PBS, 0.02% sodium azide
Purity

Monoclonal antibodies are purified by peptide affinity chromatography

ConjugationUnconjugated
MW34 kDa
TargetmCherry
StorageAt -20°C for one year. After reconstitution at 4°C for two weeks. It can also be aliquotted and stored frozen at -20°C for a longer time.
DescriptionMouse monoclonal antibody to mCherry which is a fluoro protein. Can be used as a tracer for tracking molecule pathways. Can perform several biological functions like bioluminescence and can generate energy as well as precursor metabolites.
Alternative namesanti-APRF antibody, anti-Acute-phase response factor antibody, anti-Signal transducer and activator of transcription 3 antibody(click to expand)
Expiration Date12 months from date of receipt.
NoteFor research use only.
  1. Shearin, Harold K. et al. Expansion of the gateway multisite recombination cloning toolkit PLoS One, 8, e77724 (2013)
  2. Shearin, Harold K. et al. Hexameric GFP and mCherry reporters for the Drosophila GAL4, Q, and LexA transcription systems Genetics, 196, 951-960 (2014)
Western blot analysis of  mcherry protein (lane 1) Recombinant protein using anti-mCherry (1 ug/ml)
Western blot analysis of mcherry protein (lane 1) Recombinant protein using anti-mCherry (1 ug/ml)
WB analysis of  protein 20 ng (lane 1), protein 50 ng (lane 2), protein 100 ng (lane3), protein 200 ng (lane 4) Recombinant protein using mCherry antibody (1 ug/ml)
WB analysis of protein 20 ng (lane 1), protein 50 ng (lane 2), protein 100 ng (lane3), protein 200 ng (lane 4) Recombinant protein using mCherry antibody (1 ug/ml)
  1. 3 images
    • Western blot analysis of transfected 293 HEK with mCherry cell lysate using mCherry antibody
    • Immunohistochemical analysis of mouse mammary tumor cell line using mCherry goat antibody
    • Confocal immunofluorescence analysis of COS-7 transfected with mCherry-EEA1 using mCherry antibody
    mCherry antibody [orb11618]

    IF, IHC-Fr, IHC-P, IEM, WB

    Goat

    Polyclonal

    Unconjugated

    100 μg
  2. 2 images
    • Western blot analysis of HEK293 cell line lysate using tdTomato antibody.
    • Confocal immunofluoroscence analysis of HEK293 cells using tdTomato antibody.
    tdTomato antibody [orb182397]

    IF, IHC-Fr, IHC-P, IEM, WB

    Goat

    Polyclonal

    Unconjugated

    100 μg, 600 μg
  3. 2 images
    • Western blot analysis of HEK293 cell line lysate using mCherry antibody.
    • Confocal immunofluoroscence analysis of HEK293 cells using mCherry antibody.
    mCherry antibody [orb153320]

    IF, IHC-Fr, IHC-P, IEM, WB

    Goat

    Polyclonal

    Unconjugated

    100 μg
  4. 2 images
    • Western blot analysis of recombinant mcherry antibody (dilution of primary antibody at  1:500)
    • WB analysis of recombinant mcherry antibody (dilution of primary antibody- 1:500)
    mCherry antibody [orb181783] Custom conjugation service available

    ELISA, WB

    Rabbit

    Polyclonal

    Unconjugated

    100 μg, 200 μg
  5. 2 images
    • Western blot analysis of 293T cell lysate using mCherry-tag antibody
    • Western blot analysis of HEK293T cells using mCherry-tag antibody
    mCherry-tag antibody [orb393335] Custom conjugation service available

    IP, WB

    Mouse

    Monoclonal

    30 μl, 100 μl, 200 μl