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mCherry antibody
SKU: orb66657
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Description
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| Tested Applications | ELISA, IHC-P, WB |
|---|---|
| Dilution range | WB: 1 μg/ml |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 5A12A7H10 |
| Immunogen | Human mCherry recombinant protein (orb178365) was used as immunogen. |
| Target | mCherry |
| Molecular Weight | 34 kDa |
| Purity | Monoclonal antibodies are purified by peptide affinity chromatography |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 10 mM PBS, 0.02% sodium azide |
| Concentration | - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−anti-APRF antibody, anti-Acute-phase response factor antibody, anti-Signal transducer and activator of transcription 3 antibody
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WB analysis of protein 20 ng (lane 1), protein 50 ng (lane 2), protein 100 ng (lane3), protein 200 ng (lane 4) Recombinant protein using mCherry antibody (1 ug/ml)
Western blot analysis of mcherry protein (lane 1) Recombinant protein using anti-mCherry (1 ug/ml)
Quick Database Links
Gene Symbol
mCherry
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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Luqi Zhao1 , Sana Sharif1 , Xinli Liu1 , Mingfu Wu An Innovative Method of Endosome Isolation from Mouse Heart Tissues and Co-Culture with Cardiomyocytes Journal of Visualized Experiments, (2025)
Applications
WB
Reactivity
Mouse
Shearin, Harold K. et al. Expansion of the gateway multisite recombination cloning toolkit PLoS One, 8, e77724 (2013)
Applications
IHC
Reactivity
Drosophila
mCherry antibody (orb66657)
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