| Catalog Number | orb66657 |
|---|---|
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Tested applications | ELISA, IHC-P, WB |
| Reactivity | Human, Rat |
| Clone ID | 5A12A7H10 |
| Isotype | Mouse IgG1 |
| Immunogen | Human mCherry recombinant protein (orb178365) was used as immunogen. |
| Concentration | - 100 μg (in 200 μl): 0.5 mg/ml |
| Dilution range | WB: 1 μg/ml |
| Form/Appearance | 10 mM PBS, 0.02% sodium azide |
| Purity | Monoclonal antibodies are purified by peptide affinity chromatography |
| Conjugation | Unconjugated |
| MW | 34 kDa |
| Target | mCherry |
| Storage | At -20°C for one year. After reconstitution at 4°C for two weeks. It can also be aliquotted and stored frozen at -20°C for a longer time. |
| Description | Mouse monoclonal antibody to mCherry which is a fluoro protein. Can be used as a tracer for tracking molecule pathways. Can perform several biological functions like bioluminescence and can generate energy as well as precursor metabolites. |
| Alternative names | anti-APRF antibody, anti-Acute-phase response factor antibody, anti-Signal transducer and activator of transcription 3 antibody(click to expand) |
| Expiration Date | 12 months from date of receipt. |
| Note | For research use only. |
- Shearin, Harold K. et al. Expansion of the gateway multisite recombination cloning toolkit PLoS One, 8, e77724 (2013)
- Shearin, Harold K. et al. Hexameric GFP and mCherry reporters for the Drosophila GAL4, Q, and LexA transcription systems Genetics, 196, 951-960 (2014)
Western blot analysis of mcherry protein (lane 1) Recombinant protein using anti-mCherry (1 ug/ml)
WB analysis of protein 20 ng (lane 1), protein 50 ng (lane 2), protein 100 ng (lane3), protein 200 ng (lane 4) Recombinant protein using mCherry antibody (1 ug/ml)
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- 2 imagestdTomato antibody [orb182397]
IF, IHC-Fr, IHC-P, IEM, WB
Goat
Polyclonal
Unconjugated
100 μg, 600 μg -
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