MCAM Antibody

• Catalog Number: orb153460
• Category: Antibodies
• Description: Mouse monoclonal antibody to MCAM
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 6C3E6
• Tested applications: FC, IHC, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Purified recombinant fragment of human MCAM (AA: 84-189) expressed in E. Coli.
• Antibody Type: Primary Antibody
• Concentration: Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration.
• Dilution range: WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000
• Conjugation: Unconjugated
• MW: 71.6kDa
• Target: MCAM
• Entrez: 4162
• UniProt ID: P43121
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody in PBS with 0.05% sodium azide
• Alternative names: CD146; MUC18
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HEK293 (Lane 1) HEK293 (Lane 2) cell lysate using CD146 antibody

Western blot analysis of human MCAM using CD146 antibody

Western blot analysis of HUVE-12 (Lane 1), EVC-304 (Lane 2), HELA (Lane 3) MCF-7 (Lane 4) cell lysate using CD146 antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded esophageal cancer tissues using CD146 antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded liver cancer tissues using CD146 antibody

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of CD146 antibody

Flow cytometric analysis of MCF-7 cells lysates using CD146 antibody