CD146 antibody

• Catalog Number: orb10290
• Category: Antibodies
• Description: CD146 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD146. Please contact us for the exact immunogen sequence. The peptide is available as orb12884.
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Dilution range: IF/ICC: 1:100-600, IHC-P: 1:100-600, WB: 1:200-1000
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography
• Conjugation: Unconjugated
• MW: 72 kDa
• Target: CD146
• Entrez: 4162
• UniProt ID: Q8R2Y2, P43121, Q9EPF2
• RRID: AB_10746790
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti-A32 antigen antibody, anti-CD 146 antibody, a
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of human breast cancer tissue using CD146 antibody (dilution of primary antibody - 2.5 ug/ml)

Immunohistochemical staining of mouse kidney tissue using anti-CD146 (dilution of primary antibody - 2.5 ug/ml)

Western blot analysis of mouse brain (lane 1), mouse colon (lane 2), mouse kidney (lane 3), rat spleen (lane 4), human breast cancer (lane 5), U251 cells (lane 6)

293T cells (lane 7) using CD146 antibody (1 ug/ml)

Immunohistochemical staining of rat brain tissue using CD146 antibody (dilution of primary antibody - 2.5 ug/ml)

IF analysis of mouse kidney tissue using CD146 antibody (dilution of primary antibody at 2.5 ug/ml)

WB analysis of mouse brain (lane 1), mouse colon (lane 2), mouse kidney (lane 3), rat spleen (lane 4), human breast cancer (lane 5), U251 cells (lane 6)

293T cells (lane 7) using CD146 antibody (1 ug/ml)