Cart summary

You have no items in your shopping cart.

Anti-CD146/Mcam Antibody

Catalog Number: orb654343

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb654343
CategoryAntibodies
DescriptionAnti-CD146/Mcam Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Mouse, Rat.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, FC, IF, IHC, WB
ReactivityMouse, Rat
IsotypeRabbit IgG
ImmunogenE.coli-derived mouse CD146/Mcam recombinant protein (Position: E27-E621).
Antibody TypePrimary Antibody
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form/AppearanceLyophilized
ConjugationUnconjugated
MW120 kDa
UniProt IDQ8R2Y2
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesCell surface glycoprotein MUC18; Gicerin; Melanoma
Read more...
NoteFor research use only
Application notesWestern blot, 0.1-0.25μg/ml, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat Immunofluorescence, 2μg/ml, Mouse Flow Cytometry (Fixed), 1-3μg/1x106 cells, Mouse, Rat ELISA, 0.1-0.5μg/ml, -. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
Expiration Date12 months from date of receipt.
Anti-CD146/Mcam Antibody

Flow Cytometry analysis of C6 cells using anti-MCAM antibody. Overlay histogram showing C6 cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCAM Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Anti-CD146/Mcam Antibody

Flow Cytometry analysis of Neuro-2a cells using anti-MCAM antibody. Overlay histogram showing Neuro-2a cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCAM Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Anti-CD146/Mcam Antibody

IF analysis of MCAM using anti-MCAM antibody. MCAM was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/mL rabbit anti-MCAM Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Anti-CD146/Mcam Antibody

IHC analysis of MCAM using anti-MCAM antibody. MCAM was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-MCAM Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-CD146/Mcam Antibody

IHC analysis of MCAM using anti-MCAM antibody. MCAM was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-MCAM Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-CD146/Mcam Antibody

IHC analysis of MCAM using anti-MCAM antibody. MCAM was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-MCAM Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-CD146/Mcam Antibody

Western blot analysis of MCAM using anti-MCAM antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: rat ovary tissue lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse lung tissue lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse thymus tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse ovary tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCAM antigen affinity purified polyclonal antibody at 0.25 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MCAM at approximately 120 KD. The expected band size for MCAM is at 120 KD.

  • Anti-CD146/MCAM Antibody (monoclonal, 4C12) [orb654279]

    FC,  IHC,  IHC-Fr,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    100 μg, 10 μg
  • Anti-CD146 MCAM Rabbit Monoclonal Antibody [orb547823]

    FC,  ICC,  IF,  IHC,  IP,  WB

    Human, Mouse, Rat

    Rabbit

    Monoclonal

    Unconjugated

    30 μl, 100 μl
  • Anti-CD146/MCAM Antibody (monoclonal, 2H12) [orb654280]

    IHC,  IHC-Fr,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    100 μg, 10 μg
  • Anti-CD146/MCAM Antibody [orb234277]

    IHC,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    10 μg, 100 μg
  • Anti-CD146/MCAM Antibody Picoband (monoclonal, 2H12) [orb1882284]

    IHC,  IHC-Fr,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    100 μg