MBD2 Antibody

SKU: orb1247282

Description

MBD2 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 48-50kDa band observed in lysates of cell line Jurkat (calculated MW of 43.3kDa according to NP_003918.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Tan et al, Mol Cell Biol. 2006 Oct;26(19):7224-35.; PMID: 16980624). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Colon, Adrenal Gland and Skin. Recommended concentration: 2.5ug/ml.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-RNDPLNQNKGKPDLN
• Target: MBD2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

MBD2, methyl-CpG binding domain protein 2 , DKFZp586O0821, DMTase, NY-CO-41

orb1247282 (0.3 ug/ml) staining of Jurkat cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247282 (2.5 ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247282 (2.5 ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247282 (2.5 ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

MBD2

UniProt

Q9UBB5

RefSeq (Protein):NP_003918.1