MBD2 Antibody (Center)

SKU: orb1932036

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Metabolism Research

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: IF - 1:10-50, WB - 1:1000
• Reactivity: Human
• Predicted Reactivity: Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD63885
• Immunogen: This MBD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-279 amino acids from the Central region of human MBD2. Antigen Region: 250-279 aa.
• Target: MBD2 (HGNC:6917)
• Molecular Weight: 43255 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Methyl-CpG-binding domain protein 2, Demethylase, DMTase, Methyl-CpG-binding protein MBD2, MBD2

Fluorescent confocal image of Hela cell stained with MBD2 Antibody (Center).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MBD2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).MBD2 immunoreactivity is localized to Nucleus significantly.

MBD2 Antibody (Center) western blot analysis in HL-60 cell line lysates (35 ug/lane). This demonstrates the MBD2 antibody detected the MBD2 protein (arrow).

Quick Database Links

Gene Symbol

MBD2 (HGNC:6917)

UniProt

Q9UBB5

RefSeq (Protein):NP_056647.1, NP_003918.1