MBD2 (isoform 1) Antibody

SKU: orb19388

Description

Goat polyclonal antibody to MBD2

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:64000, WB: 0.3-1 μg/ml, IHC-P: 2.5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded Human Colon shows nuclear stianing of enterocytes. Recommended concentration, 2-4ug/ml.WB: Approx 48-50kDa band observed in lysates of cell line Jurkat (calculated MW of 43.3kDa according to NP_003918.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Tan et al, Mol Cell Biol. 2006 Oct;26(19):7224-35.; PMID: 16980624). Recommended concentration: 0.3-1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: MBD2 (isoform 1)
• Molecular Weight: 43.3; 31.7
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti MBD2 antibody, anti methyl-CpG binding domain protein 2 antibody, anti DKFZp586O0821 antibody, anti DMTase antibody, anti NY-CO-41 antibody

Western blot analysis of Jurkat cell lysate using MBD2 antibody.

Immunohistochemical staining of Human Colon using MBD2 antibody.

Immunohistochemical staining of Human Adrenal Gland using MBD2 antibody.

Immunohistochemical staining of Human Skin using MBD2 antibody.

Quick Database Links

Gene Symbol

MBD2 (isoform 1)

NCBI Gene ID

8932