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MART-1 / Melan-A Antibody

Catalog Number: orb639450

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb639450
CategoryAntibodies
DescriptionThis antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. There are seven other melanoma associated antigens recognized by autologous cytotoxic T cells: MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MART-1 antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. The antibody does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
ClonalityMonoclonal
Species/HostMouse
IsotypeMouse IgG2b, lambda
ConjugationUnconjugated
ReactivityHuman
ImmunogenRecombinant human MART-1 protein was used as the immunogen for this antibody.
Tested applicationsIHC-P, WB
Dilution rangeWestern blot: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application notesThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MART-1 antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Antibody TypePrimary Antibody
Clone NumberM2-7C10
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Hazard InformationThis MART-1 antibody is available for research use only.
NoteFor research use only
MART-1 / Melan-A Antibody

IHC testing of FFPE human melanoma stained with MART-1 antibody (clone M2-7C10). Note cytoplasmic staining of cells. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.

MART-1 / Melan-A Antibody

IHC testing of FFPE human melanoma stained with MART-1 antibody (clone M2-7C10). Note cytoplasmic staining of cells. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.

MART-1 / Melan-A Antibody

IHC testing of FFPE human skin stained with MART-1 antibody (clone M2-7C10). Note cytoplasmic staining of cells. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.

MART-1 / Melan-A Antibody

SDS-PAGE Analysis of Purified, BSA-Free MART-1 Antibody (clone M2-7C10). Confirmation of Integrity and Purity of the Antibody.

MART-1 / Melan-A Antibody

Western blot testing of human COLO-38 cell lysate with MART-1 antibody (clone M2-7C10). Expected molecular weight ~20 kDa.

  • MART-1 / Melan-A Antibody [orb248349]

    FACS,  IF,  IHC-P,  WB

    Human, Mouse, Rat

    Mouse

    Monoclonal

    Unconjugated

    20 μg, 100 μg
  • MART-1 / Melan-A Antibody [orb386243]

    IHC-P,  WB

    Human

    Rabbit

    Recombinant

    Unconjugated

    20 μg, 100 μg
  • MART-1 / Melan-A Antibody [orb386244]

    IHC-P,  WB

    Human

    Rabbit

    Recombinant

    Unconjugated

    20 μg, 100 μg
  • MART-1/Melan-A Antibody (Center) [orb1938018]

    FC,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 50 μl
  • MART-1 / Melan-A Antibody [orb749497]

    IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg, 100 μg