MART-1 / Melan-A Antibody

• Catalog Number: orb639450
• Category: Antibodies
• Description: This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. There are seven other melanoma associated antigens recognized by autologous cytotoxic T cells: MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MART-1 antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. The antibody does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2b, lambda
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant human MART-1 protein was used as the immunogen for this antibody.
• Tested applications: IHC-P, WB
• Dilution range: Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MART-1 antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: M2-7C10
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only

IHC testing of FFPE human melanoma stained with MART-1 antibody (clone M2-7C10). Note cytoplasmic staining of cells. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.

IHC testing of FFPE human melanoma stained with MART-1 antibody (clone M2-7C10). Note cytoplasmic staining of cells. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.

IHC testing of FFPE human skin stained with MART-1 antibody (clone M2-7C10). Note cytoplasmic staining of cells. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.

SDS-PAGE Analysis of Purified, BSA-Free MART-1 Antibody (clone M2-7C10). Confirmation of Integrity and Purity of the Antibody.

Western blot testing of human COLO-38 cell lysate with MART-1 antibody (clone M2-7C10). Expected molecular weight ~20 kDa.