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Item 1 of 3
MART-1/Melan-A Antibody (Center)
Catalog Number: orb1938018
| Catalog Number | orb1938018 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Target | This MART-1/Melan-A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 34-60 amino acids from the Central region of human MART-1/Melan-A. |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| UniProt ID | Q16655 |
| MW | 13157 Da |
| Tested applications | FC, WB |
| Dilution range | WB: 1:1000, WB: 1:2000, FC: 1:25 |
| Antibody Type | Primary Antibody |
| Clone Number | RB19079 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Melanoma antigen recognized by T-cells 1, MART-1, Read more... |
| Note | For research use only |
| NCBI | NP_005502.1 |
Anti-MLANA Antibody (Center) at 1:2000 dilution + SK-MEL-3 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 13 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
MART-1/Melan-A Antibody (Center) western blot analysis in HL-60 cell line lysates (35 ug/lane). This demonstrates the MART-1/Melan-A antibody detected the MART-1/Melan-A protein (arrow).
Overlay histogram showing A2058 cells (green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.
