MART-1 / Melan-A Antibody

• Catalog Number: orb386243
• Category: Antibodies
• Description: MART-1/Melan-A is a protein antigen that is found on the surface of melanocytes. Antibodies against the antigen are used in the medical specialty of anatomic pathology in order to recognize cells of melanocytic differentiation, useful for the diagnosis of a melanoma. The MART-1/Melan-A antigen is specific for the melanocyte lineage, found in normal skin, the retina, and melanocytes, but not in other normal tissues. It is thus useful as a marker for melanocytic tumors (melanomas) with the caveat that it is normally found in benign nevi as well. [Wiki]
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: MLANA/1409R
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: Human protein was used as the immunogen for this recombinant MART-1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant MART-1 antibody is available for research use only.
• UniProt ID: Q16655
• Storage: Store the recombinant MART-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The optimal dilution of the recombinant MART-1 antibody for each application should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of formalin-fixed, paraffin-embedded human melanoma stained with recombinant MART-1 antibody (clone MLANA/1409R). HIER: steam sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC testing of formalin-fixed, paraffin-embedded human melanoma stained with recombinant MART-1 antibody (clone MLANA/1409R). HIER: steam sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free recombinant MART-1 antibody (clone MLANA/1409R) as confirmation of integrity and purity.

Western blot testing of human COLO-38 cell lysate with recombinant Melan-A antibody (clone MLANA/1761R). Expected molecular weight ~20 kDa with possible doublet.