MAP2K2 Antibody (S222)

SKU: orb1928727

Description

MAP2K2 Antibody (S222)

Research Area

Cancer Biology, Immunology & Inflammation, Immunology & inflammation, Signal Transduction

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Drosophila, Gallus, Hamster, Rabbit, Xenopus

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 44424 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Alternative Names

MEK2, MKK2, PRKMK2

Western blot analysis of MAP2K2-S222 in Y79 cell line lysates (35 ug/lane). MAP2K2 (arrow) was detected using the purified Pab.

Western blot analysis of MAP2K2 (arrow) using rabbit polyclonal MAP2K2-S222. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K2 gene (Lane 2).

All lanes: Anti-MAP2K2 Antibody (S222) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: MOLT-4 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 44 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with MAP2K2 Antibody (S222), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Quick Database Links

UniProt

P36507