Phospho-MEK1/2 (Ser218 + Ser222) Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb559179
• Category: Antibodies
• Description: Phospho-MEK1/2 (Ser218 + Ser222) Recombinant Rabbit Monoclonal Antibody
• Target: MAP2K2
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine, Rabbit
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human MEK1 around the phosphorylation site of Ser218/222 ID(p-S)MAN(p-S)FV
• UniProt ID: P36507
• MW: 43 kDa
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, ICC/IF=1:50-200, IF=1:50-200
• Antibody Type: Primary Antibody
• Clone Number: 8A1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MEK1(S218/S222); MEK1+MEK2(phospho S218+S222); p-M
• Research Area: Cell Biology, MAPK/ERK Signalling Pathway, Tyrosin
• Note: For research use only

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Rat Cerebrum tissue lysates, Lane 3: Human Du145 cell lysates, Lane 4: Human HepG2 cell lysates, Lane 5: Human U251 cell lysates, Lane 6: Human A549 cell cell lysates, Primary: Anti-Phospho-MEK1/2 (Ser218 + Ser222) (orb559179) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45 kDa, Observed band size: 45 kDa.