Anti-MKK1/2 (Phospho-S222/226) Antibody

• Catalog Number: orb224177
• Category: Antibodies
• Description: Rabbit polyclonal antibody to MKK1/2 (Phospho-S222/226)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IH, IP, WB
• Reactivity: Human, Mouse, Rabbit, Rat, Zebrafish
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S222/226 of human MKK1/2 protein. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-1:1000, IHC-P: 1:100-1:200, IF/ICC: 1:100-1:500, IP: 1:10-1:100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: MAP2K1; MAP2K2
• Entrez: 26395, 5604, 170851, 58960, 5605, 26396
• UniProt ID: Q63932, Q01986, P31938, P36506, Q02750, P36507
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti MAP2K1 antibody, anti MEK1 antibody, anti PRK
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of MKK1/2 (Phospho-S222/226) expression in HeLa EGF-treated (A), K562 (B) whole cell lysates. (Predicted band size: 43; 44 kD; Observed band size: 45 kD)

Immunohistochemical analysis of MKK1/2 (Phospho-S222/226) staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of MKK1/2 (Phospho-S222/226) staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).