LTF Antibody

• Catalog Number: orb1788113
• Category: Antibodies
• Description: Purified Mouse Monoclonal Antibody (Mab)
• Target: This LTF Monoclonal antibody is generated from mouse immunized with LTF recombinant protein.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1κ
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Immunogen: 249-712 aa
• UniProt ID: P02788
• MW: 78182 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: IF: 1:10~50, IF: 1:10~50, IF: 1:25, WB: 1:1000, IHC-P: 1:50~100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Lactotransferrin, Lactoferrin, 3421-, Growth-inhib
• Note: For research use only
• NCBI: NP_002334.2, NP_001186078.1

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hela (Human Cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on Hela cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).

Confocal immunofluorescent analysis of LTF Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).

Immunofluorescence analysis of LTF Monoclonal Antibody with paraffin-embedded human prostate carcinoma tissue.0.05 mg/ml primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule). PE emits orange fluorescence.

Formalin-fixed and paraffin-embedded human prostate carcinoma with LTF Monoclonal Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-LTF Antibody at 1:1000 dilution. Lane 1: K562 whole cell lysates. Lane 2: Hela whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 78 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.