LTF Antibody

SKU: orb1939426

Description

LTF Antibody

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution range: WB - 1:8000
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,κ
• Molecular Weight: 78182 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Disclaimer: For research use only

Alternative Names

GIG12, LF

Anti-LTF at 1:8000 dilution + K562 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 78 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of LTF Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human prostate carcinoma with LTF Monoclonal Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence analysis of LTF Monoclonal Antibody with paraffin-embedded human prostate carcinoma tissue. 0.05 mg/ml primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule). PE emits orange fluorescence.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hela (Human Cervical epithelial adenocarcinoma cell line) cells labeling TRFL at 1/25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on Hela cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).

Quick Database Links

UniProt

P02788