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Catalog Number | orb1928337 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Target | This LTF antibody is generated from rabbits immunized with LTF recombinant protein. |
Clonality | Polyclonal |
Species/Host | Rabbit |
Isotype | Rabbit IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
UniProt ID | P02788 |
MW | 78182 Da |
Tested applications | FC, WB |
Dilution range | WB: 1:1000, WB: 1:1000, WB: 1:4000, WB: 1:1000-2000, WB: 1:1000, FC: 1:10~50 |
Antibody Type | Primary Antibody |
Clone Number | RB22673 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Lactotransferrin, Lactoferrin, 3421-, Growth-inhib Read more... |
Note | For research use only |
NCBI | NP_002334.2, NP_001186078.1 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of LTF Antibody in MDA-MB231 cell line lysates (35 ug/lane). LTF (arrow) was detected using the purified Pab.
Western blot analysis of LTF Antibody in mouse spleen tissue lysates (35 ug/lane). LTF (arrow) was detected using the purified Pab.
LTF Antibody flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
All lanes: Anti-LTF Antibody at 1:1000-2000 dilution. Lane 1: Human plasma tissue lysate. Lane 2: Hela whole cell lysate. Lane 3: Human uterus tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 78 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-LTF Antibody at 1:1000 dilution. Lane 1: Human plasma tissue lysate. Lane 2: Human fetal kidney tissue lysate. Lane 3: Human uterus tissue lysate. Lane 4: Human skeletal muscle tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 78 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-LTF Antibody at 1:4000 dilution. Lane 1: A549 whole cell lysate. Lane 2: Human plasma tissue lysate. Lane 3: Human fetal kidney tissue lysate. Lane 4: Human breast tissue lysate. Lane 5: Human uterus tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 78 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
ELISA, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FACS, IF, IHC-P | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IF, IHC-P | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IF, IHC-P | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |