LIN28B Antibody

• Catalog Number: orb1271039
• Category: Antibodies
• Description: LIN28B Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Predicted Reactivity: Mouse
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This LIN28B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-128 amino acids from the Central region of human LIN28B.
• Concentration: batch dependent
• Dilution range: For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 27 kDa
• Target: LIN28B
• UniProt ID: Q6ZN17
• NCBI: Q6ZN17
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Protein lin-28 homolog B, Lin-28B, LIN28B, CSDD2
• Note: For research use only
• Application notes: For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000
• Expiration Date: 12 months from date of receipt.

Antibody staining LIN28B in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Antibody staining LIN28B in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Western Blot at 1:2000 dilution + NCCIT whole cell lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lane 3: mouse testis lysate Lane 4: NCCIT whole cell lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:1000 dilution Lane 1: HepG2 whole cell lysates Lane 2: K562 whole cell lysates Lane 3: NCCIT whole cell lysates Lysates/proteins at 20 ug per lane.

Western Blot at 1:1000 dilution Lane 1: HepG2 whole cell lysates Lane 2: K562 whole cell lysates Lysates/proteins at 20 ug per lane.

Western blot analysis of anti-LIN28B Pab in K562 cell line lysates (35 ug/lane).