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LIN28B Antibody
SKU: orb1271039
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Description
Images & Validation
−Item 1 of 7
| Tested Applications | FC, IF, IHC-P, WB |
|---|---|
| Reactivity | Human |
| Predicted Reactivity | Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit Ig |
| Immunogen | This LIN28B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-128 amino acids from the Central region of human LIN28B. |
| Target | LIN28B |
| Molecular Weight | 27 kDa |
| Purification | This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−Protein lin-28 homolog B, Lin-28B, LIN28B, CSDD2
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Antibody staining LIN28B in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Antibody staining LIN28B in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Western Blot at 1:2000 dilution + NCCIT whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lane 3: mouse testis lysate Lane 4: NCCIT whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000 dilution Lane 1: HepG2 whole cell lysates Lane 2: K562 whole cell lysates Lane 3: NCCIT whole cell lysates Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000 dilution Lane 1: HepG2 whole cell lysates Lane 2: K562 whole cell lysates Lysates/proteins at 20 ug per lane.
Western blot analysis of anti-LIN28B Pab in K562 cell line lysates (35 ug/lane).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
IF
Immunofluorescence
LIN28B Antibody (orb1271039)
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