LIN28B Rabbit Polyclonal Antibody

• Catalog Number: orb1841
• Category: Antibodies
• Description: LIN28B Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Equine, Gallus, Mouse, Porcine, Rabbit, Sheep
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Lin-28B (65-160/250aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:50-200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 27 kDa
• Target: LIN28B
• UniProt ID: Q6ZN17
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Lin-28B; CSDD 2; CSDD2; FLJ16517; Lin 28 homolog B
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Raji Cell (Human) Lysate at 40 ug, Primary: Anti-Lin-28B (orb1841) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 27 kD, Observed band size: 27 kD.

Tissue/Cell: rat placenta tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Lin-28B Polyclonal Antibody, Unconjugated (orb1841) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: rat testis tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Lin-28B Polyclonal Antibody, Unconjugated (orb1841) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.