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Catalog Number | orb101823 |
---|---|
Category | Antibodies |
Description | LGALS3BP Rabbit Polyclonal Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC-Fr, IHC-P, WB |
Predicted Reactivity | Mouse, Rat |
Reactivity | Human |
Isotype | IgG |
Immunogen | KLH conjugated synthetic peptide derived from human LGALS3BP (351-450/585aa) |
Antibody Type | Primary Antibody |
Concentration | 1mg/ml |
Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 63 kDa |
Target | LGALS3BP |
UniProt ID | Q08380 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Alternative names | Mac 2 binding protein; MAC2BP; Tumor associated an Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Sample: Heart (Mouse) Lysate at 40 ug, Heart (Rat) Lysate at 40 ug, Primary: Anti-LGALS3BP (orb101823) at 1/2000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 63 kD, Observed band size: 63 kD.
Sample: Lane 1: Mouse Heart tissue lysates, Lane 2: Mouse Kidney tissue lysates, Lane 3: Rat Heart tissue lysates, Lane 4: Rat Kidney tissue lysates, Primary: Anti-LGALS3BP (orb101823) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 63 kD, Observed band size: 78 kD.
Tissue/cell: human colon carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-LGALS3BP Polyclonal Antibody, Unconjugated (orb101823) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
IP, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Bovine, Canine, Mouse, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |