LGALS3BP Antibody (Center)

SKU: orb1932111

Description

LGALS3BP Antibody (Center)

Research Area

Cancer Biology, Signal Transduction

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution range: WB - 1:1000-1:2000
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 65331 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Alternative Names

M2BP

Immunohistochemical analysis on paraffin-embedded Human prostate tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 15 min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary antibody.

All lanes: Anti-LGALS3BP Antibody (Center) at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: Human plasma lysate. Lane 3: Mouse lung lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 65 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-LGALS3BP Antibody (Center) at 1:2000 dilution. Lane 1: Human liver tissue lysate. Lane 2: Mouse lung tissue lysate. Lane 3: Human plasma tissue lysate. Lane 4: NIH/3T3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 65 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

Q08380