LGALS3BP Antibody (Center)

• Catalog Number: orb1932111
• Category: Antibodies
• Description: LGALS3BP Antibody (Center)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q08380
• MW: 65331 Da
• Tested applications: IHC-P, WB
• Dilution range: WB - 1:1000-1:2000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: M2BP
• Research Area: Cancer Biology, Signal Transduction
• Note: For research use only

Immunohistochemical analysis on paraffin-embedded Human prostate tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 15 min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary antibody.

All lanes: Anti-LGALS3BP Antibody (Center) at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: Human plasma lysate. Lane 3: Mouse lung lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 65 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-LGALS3BP Antibody (Center) at 1:2000 dilution. Lane 1: Human liver tissue lysate. Lane 2: Mouse lung tissue lysate. Lane 3: Human plasma tissue lysate. Lane 4: NIH/3T3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 65 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.