LAG3 Antibody

SKU: orb1239550

Description

LAG3 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ICC, IF, IHC-P
• Reactivity: Human
• Application Notes:
  LAG-3 antibody can be used for Immunocytochemistry start at 1 μg/mL. For Immunofluorescence start at 10 μg/mL. For Immunohistochemistry start at 5 μg/mL. Antibody validated: Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone No.: 6D1
• Immunogen: LAG-3 antibody was raised against the extracellular domain of human LAG-3
• Target: LAG3
• Purification: LAG-3 Antibody is supplied as protein A purified IgG1.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: LAG-3 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

LAG3 Antibody: lymphocyte activating 3, LAG-3, CD223

Immunocytochemistry of LAG-3 in over expressing HEK293 cells using LAG-3 antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.

Immunofluorescence of LAG-3 in over expressing HEK293 cells using LAG-3 Antibody at 2 μg/ml. Green: LAG3 Antibody [6D1] (orb1239550) Blue: DAPI staining

Immunofluorescence of LAG-3 in over human spleen tissue using LAG-3 Antibody at 10 μg/ml. Green: LAG3 Antibody [6D1] (orb1239550) Blue: DAPI staining

Immunohistochemistry of LAG-3 in human lymphoma tissue using LAG-3 Antibody and control mouse IgG (corner box) at 5 μg/ml.

Flow cytometry analysis of LAG-3 over expressing HEK293 cells using LAG-3 antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: LAG-3 over expressing HEK293 cells.

Titration curve analysis of LAG-3 mAbs to detect recombinant LAG-3 in ELISA with orb1239547, orb1239545, orb1239551, orb1239549, orb1239550, orb1239548, and orb1239546 antibodies at decreasing concentrations.

Quick Database Links

Gene Symbol

LAG3

UniProt

P18627

RefSeq (Protein):NP_002277