LAG3 Antibody [2G8]

• Catalog Number: orb1239547
• Category: Antibodies
• Description: LAG3 Antibody [2G8]
• Clonality: Monoclonal
• Clone Number: 2G8
• Tested applications: ELISA, FC, ICC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1,k
• Immunogen: LAG-3 antibody was raised against the extracellular domain of human LAG-3
• Antibody Type: Primary Antibody
• Concentration: 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 55 kDa Observed: 60, 70, 80 kDa
• Target: LAG3
• UniProt ID: P18627
• NCBI: NP_002277
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: LAG-3 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
• Alternative names: LAG3 Antibody: lymphocyte activating 3, LAG-3, CD2
• Note: For research use only
• Application notes: LAG-3 antibody can be used for ELISA starting at 0.25 μg/mL. For Western blot start at 0.25 μg/mL. For Immunocytochemistry start at 1 μg/mL. For Immunofluorescence start at 10 μg/mL. For Immunohistochemistry start at 5 μg/mL. For Flow Cytometry start at 1 μg/ml.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of LAG-3 in over expressing HEK293 cells using LAG-3 antibody at (A) 0.25 (B) 0.5 and (C) 1 μg/ml.

Immunocytochemistry of LAG-3 in over expressing HEK293 cells using LAG-3 antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.

Immunofluorescence of LAG-3 in over expressing HEK293 cells using LAG-3 Antibody at 2 μg/ml. Green: LAG3 Antibody [2G8] (orb1239547) Blue: DAPI staining

Immunofluorescence of LAG-3 in over human spleen tissue using LAG-3 Antibody at 10 μg/ml. Red: LAG3 Antibody [2G8] (orb1239547) Blue: DAPI staining

Immunohistochemistry of LAG-3 in human lymphoma tissue using LAG-3 Antibody and control mouse IgG (corner box) at 5 μg/ml.

Flow cytometry analysis of LAG-3 over expressing HEK293 cells using LAG-3 antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: LAG-3 over expressing HEK293 cells.

Titration curve analysis of LAG-3 mAbs to detect recombinant LAG-3 in ELISA with orb1239547, orb1239545, orb1239551, orb1239549, orb1239550, orb1239548, and orb1239546 antibodies at decreasing concentrations.

A sandwich ELISA was performed using the anti-LAG3 mAbs orb1239547, orb1239545, orb1239551, orb1239549, orb1239550 and orb1239548 as the capture antibodies for the LAG3 extracellular domain antigen with biotin-labeled Risk-Free anti-LAG3 mAbs as the detection antibodies.