LAG3 Antibody (Center)

• Catalog Number: orb1929672
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This LAG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-132 amino acids from the Central region of human LAG3.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: P18627
• MW: 57449 Da
• Tested applications: FC, IHC-P, WB
• Dilution range: WB: 1:2000, WB: 1:2000, WB: 1:2000, WB: 1:2000, WB: 1:1000, WB: 1:2000, WB: 1:2000
• Antibody Type: Primary Antibody
• Clone Number: RB21775
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Lymphocyte activation gene 3 protein, LAG-3, Prote
• Note: For research use only
• NCBI: NP_002277.4

Anti-LAG3 Antibody (Center) at 1:2000 dilution + mouse heart lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 57 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-LAG3 Antibody (Center) at 1:2000 dilution + K562 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 57 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-LAG3 Antibody (Center) at 1:2000 dilution + A2058 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 57 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-LAG3 Antibody (Center) at 1:2000 dilution + 293T/17 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 57 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-LAG3 Antibody (Center) at 1:1000 dilution. Lane 1: human ovary lysate. Lane 2: mouse liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 57 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-LAG3 Antibody (Center) at 1:2000 dilution. Lane 1: 293T/17 whole cell lysate. Lane 2: mouse heart lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 57 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-LAG3 Antibody (Center) at 1:2000 dilution. Lane 1: 293T/17 whole cell lysates. Lane 2: human spleen lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 57 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.