XRCC5/Ku80 Antibody

SKU: orb1151776

Description

Mouse monoclonal antibody to Ku80

Research Area

Cancer, DNA Damage, Epigenetics, Immunology, Infectious Diseases

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the XRCC5/Ku80 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: XRCC5/7315
• Immunogen: A portion of amino acids 300-500 was used as the immunogen for the XRCC5/Ku80 antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot testing of human 1) MOLT-4 and 2) HEK293 cell lysate using XRCC5/Ku80 antibody (clone XRCC5/7315). Predicted molecular weight: 80-86 kDa.

IHC staining of FFPE human lymph node tissue with XRCC5/Ku80 antibody (clone XRCC5/7315). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human salivary gland with XRCC5/Ku80 antibody (clone XRCC5/7315). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colon carcinoma tissue with XRCC5/Ku80 antibody (clone XRCC5/7315). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Quick Database Links

UniProt

P13010