XRCC5/Ku80 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb1151974
• Category: Antibodies
• Description: XRCC5/Ku80 Recombinant Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: K8C2
• Tested applications: ICC, IF, IHC-Fr, IHC-P
• Predicted Reactivity: Mouse
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human XRCC5/Ku80
• Antibody Type: Recombinant Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:1000, IHC-F=1:50-100, ICC/IF=1:100, IF=1:50-100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 83 kDa
• Target: XRCC5
• UniProt ID: P13010
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: 86 kDa subunit of Ku antigen; ATP dependent DNA he
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Cell line: HeLa, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1151974.

Cell line: NIH/3T3, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1151974.

HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (XRCC5/Ku80) monoclonal Antibody, Unconjugated (orb1151974) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (XRCC5/Ku80) Monoclonal Antibody, Unconjugated (orb1151974) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (XRCC5/Ku80) Monoclonal Antibody, Unconjugated (orb1151974) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Tissue:Mouse colon, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1151974.

Tissue:Mouse stomach, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1151974.