XRCC5 Antibody (Center K439)

SKU: orb1937835

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: FC, IF, WB
• Dilution Range: IF - 1:10-50, WB - 1:1000, FC - 1:10-50
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This XRCC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 424-450 amino acids from the Central region of human XRCC5. Antigen Region: 424-450 aa.
• Target: XRCC5
• Molecular Weight: 82705 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

X-ray repair cross-complementing protein 5, 364-, 86 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 2, ATP-dependent DNA helicase II 80 kDa subunit, CTC box-binding factor 85 kDa subunit, CTC85, CTCBF, DNA repair protein XRCC5, Ku80, Ku86, Lupus Ku autoantigen protein p86, Nuclear factor IV, Thyroid-lupus autoantigen, TLAA, X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining), XRCC5, G22P2

All lanes: Anti-XRCC5Antibody (CenterK439) at 1:1000 dilution. Lane 1: A549 whole cell lysate. Lane 2: Hela whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 83 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of XRCC5 Antibody (Center K439) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

XRCC5 Antibody (Center K439) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

XRCC5 Antibody (CenterK439) western blot analysis in human placenta tissue lysates (35 ug/lane). This demonstrates the XRCC5 antibody detected the XRCC5 protein (arrow).

Quick Database Links

Gene Symbol

XRCC5

UniProt

P13010

RefSeq (Protein):NP_066964.1