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Item 1 of 4
Item 1 of 4
XRCC5 Antibody (Center K439)
Catalog Number: orb1937835
| Catalog Number | orb1937835 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB17385 |
| Tested applications | FC, IF, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Dilution range | IF: 1:10~50, WB: 1:1000, WB: 1:1000, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 82705 Da |
| Target | This XRCC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 424-450 amino acids from the Central region of human XRCC5. |
| UniProt ID | P13010 |
| NCBI | NP_066964.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | X-ray repair cross-complementing protein 5, 364-, Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Confocal immunofluorescent analysis of XRCC5 Antibody (Center K439) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
XRCC5 Antibody (Center K439) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
XRCC5 Antibody (CenterK439) western blot analysis in human placenta tissue lysates (35 ug/lane). This demonstrates the XRCC5 antibody detected the XRCC5 protein (arrow).
All lanes: Anti-XRCC5Antibody (CenterK439) at 1:1000 dilution. Lane 1: A549 whole cell lysate. Lane 2: Hela whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 83 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
