Ku80 antibody [N3C2], Internal

• Catalog Number: orb556650
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Ku80
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC-P, IP, PLA, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Ku80. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Concentration: 1.01 mg/ml
• Dilution range: WB: 1:500-1:3000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000 , IP: 1:100-1:500
• Form/Appearance: Liquid: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Purity: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• MW: 83
• Target: Ku80
• UniProt ID: P13010
• Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles.
• Buffer/Preservatives: 0.025% ProClin 300. 1XPBS (pH7), 1% rAlbumin, 20% Glycerol
• Alternative names: X-ray repair cross complementing 5 , KARP-1 , KARP
• Note: For research use only
• Application notes: Positive control: Neuro2A , PC-12 , Rat2
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of HeLa cells using Ku80 antibody

Immunohistochemical staining of Cal27 Xenograft using Ku80 antibody

Immunoprecipitation analysis of HeLa Whole cell lysate using Ku80 antibody

Western blot analysis of Various whole cell using Ku80 antibody

Western blot analysis of Whole cell extract using Ku80 antibody

Western blot analysis of 293T whole cell lysate(Lane 1), A431 whole cell lysate(Lane 2), HeLa whole cell lysate(Lane 3), HepG2 whole cell lysate(Lane 4) using Ku80 antibody