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Catalog Number | orb1247326 |
---|---|
Category | Antibodies |
Description | KPNA4 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Predicted Reactivity | Bovine, Canine |
Reactivity | Human, Mouse, Rat |
Immunogen | The immunogen for this antibody is: C-NSSANVPTEGFQF |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 55kDa band observed in Human Testis and Ovary and approx. 60kDa in Rat Testis and Ovary and Mouse Testis lysates (calculated MW of 57.9kDa according to Human NP_002259.1, Mouse NP-032493.1 and Rat NP_001014793.1). Recommended concentration: 0.5-1ug/ml. Primary incubation 1 hour at room temperature. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | KPNA4 |
UniProt ID | O00629 |
NCBI | NP_002259.1 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | KPNA4, IPOA3, QIP1, SRP3, MGC12217, MGC26703, kary Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 55kDa band observed in Human Testis and Ovary and approx. 60kDa in Rat Testis and Ovary and Mouse Testis lysates (calculated MW of 57.9kDa according to Human NP_002259.1, Mouse NP-032493.1 and Rat NP_001014793.1). Recommended concentration: 0.5-1ug/ml. Primary incubation 1 hour at room temperature. |
Expiration Date | 12 months from date of receipt. |
orb1247326 (1 ug/ml) staining of Human Testes (A), Human Ovary (B), Mouse Testes (C), Rat Testes (D) and Rat Ovary (E) lysate (RIPA buffer, 35 ug total protein per lane). Detected by chemiluminescence.
orb1247326 (3 ug/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining. This data is from a previous batch, not on sale.
orb1247326 (5 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
ELISA, WB | |
Bovine, Canine, Human, Mouse, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
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