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Item 1 of 4
Item 1 of 4
Goat anti-KPNA4 / IPOA3 Antibody
Catalog Number: orb18608
| Catalog Number | orb18608 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to KPNA4 |
| Target | KPNA4 / IPOA3 |
| Clonality | Polyclonal |
| Species/Host | Goat |
| Conjugation | Unconjugated |
| Reactivity | Bovine, Canine, Human, Mouse, Rat |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Protein Sequence | NSSANVPTEGFQF |
| RRID | AB_10748814 |
| MW | 57.9 |
| Tested applications | ELISA, WB |
| Dilution range | ELISA: 1:128000, WB: 0.5-1 μg/ml |
| Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Testis, shows strong nuclear staining of some cells in the seminiferous tubules. Recommended concentration, 3ug/ml. In paraffin embedded Human Small Intestine shows pixulate staining in nuclei of enterocytes. Recommended concenWB: Approx 55-60kDa band observed in Human Testis and Ovary lysates (predicted size of approx. 58kDa according to NP_002259.1). Recommended for use at 0.2-1 μg/ml |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Alternative names | anti KPNA4 antibody, anti IPOA3 antibody, anti QIP Read more... |
| Note | For research use only |
| Entrez | 3840 |
orb18608 staining (0.5ug/ml) of Human Testis lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
orb18608 (3ug/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
orb18608 (5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western blot analysis of Human Testes (Lane 1), Human Ovary (Lane 2), Mouse Testes (Lane 3), Rat Testes (Lane 4) and Rat Ovary (Lane 5) lysate using KPNA4 antibody
