KPNA4/IPOA3 Antibody

SKU: orb18608

Description

Goat polyclonal antibody to KPNA4

Research Area

Epigenetics, Infectious Diseases

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:128000, WB: 0.5-1 μg/ml
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Testis, shows strong nuclear staining of some cells in the seminiferous tubules. Recommended concentration, 3ug/ml. In paraffin embedded Human Small Intestine shows pixulate staining in nuclei of enterocytes. Recommended concenWB: Approx 55-60kDa band observed in Human Testis and Ovary lysates (predicted size of approx. 58kDa according to NP_002259.1). Recommended for use at 0.2-1 μg/ml

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: KPNA4 / IPOA3
• Protein Sequence: NSSANVPTEGFQF
• Molecular Weight: 57.9
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti KPNA4 antibody, anti IPOA3 antibody, anti QIP1 antibody, anti SRP3 antibody, anti MGC12217 antibody, anti MGC26703 antibody, anti karyopherin alpha 4 (importin alpha 3) antibody, anti importin alpha 3 antibody, anti importin-alpha-Q1 antibody, anti karyopherin alpha 4 antibody

orb18608 staining (0.5ug/ml) of Human Testis lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb18608 (3ug/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

orb18608 (5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western blot analysis of Human Testes (Lane 1), Human Ovary (Lane 2), Mouse Testes (Lane 3), Rat Testes (Lane 4) and Rat Ovary (Lane 5) lysate using KPNA4 antibody

Quick Database Links

Gene Symbol

KPNA4 / IPOA3

NCBI Gene ID

3840