Goat anti-KPNA4 / IPOA3 Antibody

• Catalog Number: orb18608
• Category: Antibodies
• Description: Goat polyclonal antibody to KPNA4
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:128000, WB: 0.5-1 μg/ml
• Conjugation: Unconjugated
• MW: 57.9
• Target: KPNA4 / IPOA3
• Entrez: 3840
• Protein Sequence: NSSANVPTEGFQF
• RRID: AB_10748814
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti KPNA4 antibody, anti IPOA3 antibody, anti QIP
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Testis, shows strong nuclear staining of some cells in the seminiferous tubules. Recommended concentration, 3ug/ml. In paraffin embedded Human Small Intestine shows pixulate staining in nuclei of enterocytes. Recommended concenWB: Approx 55-60kDa band observed in Human Testis and Ovary lysates (predicted size of approx. 58kDa according to NP_002259.1). Recommended for use at 0.2-1 μg/ml
• Expiration Date: 12 months from date of receipt.

orb18608 staining (0.5ug/ml) of Human Testis lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb18608 (3ug/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

orb18608 (5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western blot analysis of Human Testes (Lane 1), Human Ovary (Lane 2), Mouse Testes (Lane 3), Rat Testes (Lane 4) and Rat Ovary (Lane 5) lysate using KPNA4 antibody