IRF5 Antibody

SKU: orb1247957

Description

IRF5 Antibody

Research Area

Cell Biology, Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in Human Spleen and Peripheral Blood Lymphocytes lysates.The calculated molecular size is 57.8kDa according to NP_001092099.1. Recommended concentration: 0.1-0.3µg/mL. Primary incubation was 1 hour.Immunohistochemistry:This antibody showed nuclear staining in Human Cervix, as confirmed by customer (data not shown). This data is from a previous batch, not on sale.Chromatin immunoprecipitation: This antibody has been successfully used in human monocyte-derived dendritic cells.This data is from a previous batch, not on sale.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-QGPWPMHPAGMQ
• Target: IRF5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IRF5, interferon regulatory factor 5, SLEB10

orb1247957 staining (1 ug/ml) Human Spleen (A) and Peripheral Blood Lymphocytes (B) lysates (RIPA buffer, 30 ug total protein per lane). Detected by chemiluminescence.

Quick Database Links

Gene Symbol

IRF5

UniProt

Q13568

RefSeq (Protein):NP_001092099.1, NP_002191.1, NP_001092097.1, NP_116032.1