IRF5 Antibody

• Catalog Number: orb18401
• Category: Antibodies
• Description: Goat polyclonal antibody to IRF5
• Target: IRF5
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Bovine, Human, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: QGPWPMHPAGMQ
• RRID: AB_10755398
• MW: 56.0; 56.0; 57.8
• Tested applications: ELISA, IHC, WB
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx. 55+60kDa bands observed in Human Spleen lysates, approx. 65kDa in lysates of cell line A549, and approx. 70kDa in lysates of cell lines Daudi (calculated MW of 57.8kDa according to NP_001092099.1). These molecular weights have been observed by other commercial sources, and were successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-2µg/ml. Primary incubation 1 hour at room temperature. IHC: Paraffin embedded Human Spleen. Recommended concentration: 5µg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti IRF5 antibody, anti interferon regulatory fac
• Note: For research use only
• Entrez: 3663

Primary incubation 1 hour at room temperature. Image A: Human Spleen lysate at primary Ab concentration 1 ug/ml. (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.

5 µg/ml staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.

Primary incubation 1 hour at room temperature. Images A+B: A549 cell lysate + peptide, incubation at primary Ab concentration 1 ug/ml and C+D: Daudi cell lysate + peptide, incubation at primary Ab concentration 1 ug/ml. (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.

Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.