IRF3 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb500087
• Category: Antibodies
• Description: IRF3 Recombinant Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: 4C3
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant human IRF3 (50-150aa)
• Antibody Type: Recombinant Antibody
• Concentration: 1mg/1ml
• Dilution range: WB=1:1000-5000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 47 kDa
• Target: IRF3
• UniProt ID: Q14653
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Interferon regulatory factor 3; IRF-3; IRF 3; IRF3
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

25 ug total protein per lane of various lysates probed with IRF3 monoclonal antibody, unconjugated (orb500087) at 1:3000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with IRF3 Monoclonal Antibody, Unconjugated (orb500087) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with IRF3 Monoclonal Antibody, Unconjugated (orb500087) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with IRF3 Monoclonal Antibody, Unconjugated (orb500087) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Heart, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with IRF3 Monoclonal Antibody, Unconjugated (orb500087) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with IRF3 Monoclonal Antibody, Unconjugated (orb500087) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Pancreas, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with IRF3 Monoclonal Antibody, Unconjugated (orb500087) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with IRF3 Monoclonal Antibody, Unconjugated (orb500087) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Tonsil, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with IRF3 Monoclonal Antibody, Unconjugated (orb500087) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Testicles, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with IRF3 Monoclonal Antibody, Unconjugated (orb500087) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.