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Phospho-IRF3 (Ser386) Recombinant Rabbit Monoclonal Antibody
Catalog Number: orb559184
| Catalog Number | orb559184 |
|---|---|
| Category | Antibodies |
| Description | Phospho-IRF3 (Ser386) Recombinant Rabbit Monoclonal Antibody |
| Species/Host | Rabbit |
| Clonality | Recombinant |
| Clone Number | 28C3 |
| Tested applications | ICC, IF, IHC-Fr, IHC-P, WB |
| Predicted Reactivity | Bovine, Canine, Mouse, Porcine, Rabbit, Rat, Sheep |
| Reactivity | Human |
| Isotype | IgG |
| Immunogen | KLH conjugated synthesised phosphopeptide derived from human IRF3 around the phosphorylation site of Ser386 S(p-S)LE |
| Antibody Type | Recombinant Antibody |
| Concentration | 1mg/ml |
| Dilution range | WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:50-200, ICC/IF=1:50, IF=1:50-200 |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | 47 kDa |
| Target | IRF3 |
| UniProt ID | Q14653 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Alternative names | IRF3(S386); IRF3(phospho S386); IRF3(phospho Ser38 Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
MCF7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Phospho-IRF3 (S386)) monoclonal Antibody, Unconjugated (orb559184) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
