STING1 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb1173056
• Category: Antibodies
• Description: STING1 Recombinant Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: H9B4
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Human
• Reactivity: Human
• Isotype: IgG
• Antibody Type: Recombinant Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-1:1000, IHC-P=1:100-1:500, IHC-F=1:100-500, ICC/IF=1:20-50, IF=0
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: STING1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Endoplasmic reticulum interferon stimulator; Media
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Lysate: THP-1, Raw264.7, HL-60, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 42 kDa, Observed MW: 36 kDa.

Cell line: THP-1, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 1 hour at room temperature, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1173056.

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (STING1) Monoclonal Antibody, Unconjugated (orb1173056) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (STING1) Monoclonal Antibody, Unconjugated (orb1173056) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Tissue: Human colon cancer, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1173056.

Tissue: Human tonsil, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1173056.