IL2 Mouse Monoclonal Antibody

SKU: orb2960427

Description

IL2 Mouse Monoclonal Antibody is an unconjugated monoclonal antibody. It shows reactivity with Human samples. It is suitable for use in ELISA, FC, NeA applications. The antibody is supplied in liquid form. It is Protein A/G purified from cell culture supernatant. Purity is >95% as determined by SDS-PAGE.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, FC, NeA
• Reactivity: Human

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: BKX2242
• Purity: >95% as determined by SDS-PAGE.
• Purification: Protein A/G purified from cell culture supernatant.
• Endotoxin: Please contact with the lab for this information.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M PBS, pH 7.4.
• Concentration: Lot dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IL2, IL-2, Aldesleukin, Interleukin-2, TCGF, T-cell growth factor

Flow-cytometry using anti-human IL2 antibody. THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL2 antibody monoclonal antibody (Catalog # orb2960427, Green Histogram) at a concentration of 5 µg/mL for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using FITC anti-human IL2 antibody. THP-1 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human IL2 monoclonal antibody (Catalog orb2960427, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using PE anti-human IL2 antibody. THP-1 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human IL2 monoclonal antibody (Catalog orb2960427, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for InVivoMAb Anti-Human IL2.

Western blot analysis was performed using anti-human IL2 monoclonal antibody at 1 µg/mL on various samples. Lane 1: recombinant human IL2 (Catalog No: orb2968277), Lane 2: negative control.

Quick Database Links

UniProt

P60568