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Anti-Hu CD25 PerCP
Catalog Number: orb154535
| Catalog Number | orb154535 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody against CD25 conjugated to PerCP |
| Clonality | Monoclonal |
| Clone Number | MEM-181 |
| Tested applications | FC |
| Reactivity | Human |
| Isotype | Mouse IgG1 |
| Immunogen | PHA-activated peripheral blood leucocytes |
| Antibody Type | Primary Antibody |
| Purity | Purified antibody is conjugated with activated Peridinin-Chlorophyll Protein (PerCP) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Conjugation | PerCP |
| Target | CD25 |
| Entrez | 3559 |
| UniProt ID | P01589 |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Alternative names | anti CD25 antibody (PerCP), anti CD 25 antibody (P Read more... |
| Note | For research use only |
| Application notes | Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
| Expiration Date | 12 months from date of receipt. |
Separation of CD25 positive cells stained using anti-human CD25 (MEM-181) PerCP antibody (10 μl reagent per million cells in 100 μl of cell suspension, red-filled) from cells stained using mouse IgG1 isotype control (MOPC-21) PerCP antibody (concentration in sample 3 μg/ml, same as CD25 PerCP concentration, black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral blood mononuclear cells.
