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Anti-Hu CD25 FITC
Catalog Number: orb44157
| Catalog Number | orb44157 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody against CD25 conjugated to FITC |
| Target | CD25 |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Conjugation | FITC |
| Reactivity | Human |
| Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Purity | Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Purification | Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Immunogen | PHA-activated peripheral blood leucocytes |
| UniProt ID | P01589 |
| RRID | AB_10990815 |
| Tested applications | FC |
| Application notes | Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. |
| Antibody Type | Primary Antibody |
| Clone Number | MEM-181 |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Alternative names | anti CD25 antibody (FITC), anti CD 25 antibody (FI Read more... |
| Note | For research use only |
| Entrez | 3559 |
Flow cytometry analysis (surface staining) of human PBMC with anti-human CD25 (MEM-181) FITC. The mononuclear cells were isolated from human peripheral blood, devided in aliquots for duplicate analysis and stimulated with PHA for 2 days. Panel A, C - staining with anti-human CD25 (MEM-181); Panel B, D - staining with a standard anti-CD25 monoclonal antibody.
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