Insulin Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb1172440
• Category: Antibodies
• Description: Insulin Recombinant Rabbit Monoclonal Antibody
• Target: INS
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human Insulin
• UniProt ID: P01308
• MW: 12 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:1000-3000, IHC-F=1:1000-3000, IF=1:1000-3000
• Antibody Type: Recombinant Antibody
• Clone Number: 2F3
• CAS Number: 11061-68-0
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ILPR; INS; Insulin A chain; Insulin B chain; IRDN;
• Note: For research use only

(Negative control) Paraformaldehyde-fixed, paraffin embedded Human Liver, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Insulin Monoclonal Antibody, Unconjugated (orb1172440) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Pancreas, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Insulin Monoclonal Antibody, Unconjugated (orb1172440) at 1:1000 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Insulin Monoclonal Antibody, Unconjugated (orb1172440) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Pancreas, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Insulin Monoclonal Antibody, Unconjugated (orb1172440) at 1:2000 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.