Rabbit anti-CTLA4 Recombinant Monoclonal Antibody

• Catalog Number: orb1784584
• Category: Antibodies
• Description: Rabbit anti-CTLA4 Recombinant Monoclonal Antibody
• Target: CTLA4
• Clonality: Recombinant
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Whole IgG
• Concentration: 100 µg/ml
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Purity: Recombinant antibody was purified from cell culture supernatant
• Immunogen: Between 173 and C-terminus
• UniProt ID: P16410
• Tested applications: IF, IHC, WB
• Dilution range: Immunohistochemistry (IHC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Multiplex Immunofluorescence (mIF) 1:20. Western Blot (WB) 1:1000
• Application notes: All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight
• Antibody Type: Primary Antibody
• Clone Number: BLR257L
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: GSE; ligand and transmembrane spliced cytotoxic T
• Background: CTLA4 is a member of the immunoglobulin superfamil
• Note: For research use only

Detection of human CTLA4 by western blot. Samples: Whole cell lysate (50 µg) from A2058, NCI-H520, MJ with 10ng/ml PMA + 500ng/ml Ionomycin for 24hrs, My-La CD4+, and NK-92 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CTLA4 recombinant monoclonal antibody (orb1784584) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human CTLA4 by immunohistochemistry. Sample: FFPE section of human appendix. Antibody: Rabbit anti-CTLA4 recombinant monoclonal antibody (orb1784584). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human CTLA4 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-CTLA4 recombinant monoclonal antibody (orb1784584). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human CTLA4 (magenta) by immunofluorescence. Sample: FFPE section of metastatic lymph node. Antibody: Rabbit anti-CTLA4 recombinant monoclonal antibody (orb1784584) used at 1:20. Secondary: Invitrogen Goat anti-rabbit IgG Alexa Fluor™ Plus 647 1:400. Counterstain: DAPI (blue).