iNOS Rabbit Polyclonal Antibody

SKU: orb1294305

Description

Rabbit polyclonal antibody to iNOS

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: Western Blot 1:1000, Immunofluorescence 1:200-1:500, Immunohistochemistry (Paraffin) 1:100
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the N-terminus region.
• Target: iNOS / NOS2
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Anti-iNOS antibody - at 1/1000 dilution. Lysates at 50 µg per lane. This blot was produced using a 5% SDS-PAGE. NC membrane was then blocked for an hour before being incubated with orb1294305 overnight at 4°C.

Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with orb1294305 anti iNOS antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with orb1294305 anti iNOS antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded mouse testis labeling iNOS with orb1294305 at 1/250.

Immunohistochemical analysis of paraffin embedded mouse testis labeling iNOS with orb1294305 at 1/250.

Quick Database Links

Gene Symbol

iNOS / NOS2