iNOS antibody

• Catalog Number: orb13614
• Category: Antibodies
• Description: iNOS antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human iNOS. Please contact us for the exact immunogen sequence. The peptide is available as orb374865.
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Dilution range: IHC-P: 1:100-600, WB: 1:100-1500
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography
• Conjugation: Unconjugated
• MW: 131 kDa
• Target: iNOS
• Entrez: 4843
• UniProt ID: P35228, P29477, Q06518
• NCBI: 31, 000625, 000616, 41
• RRID: AB_10749025
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti HEP NOS antibody, anti HEP-NOS antibody, anti
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Ayantika Kundu,Pratiti Ghosh, Biswadev Bishayi Vitexin along with verapamil downregulates efflux pump P-glycoprotein in macrophages and potentiate M1 to M2 switching via TLR4-NF-κB-TNFR2 pathway in lipopolysaccharide treated mice Immunobiology, 229(1), 152767 (2024)

PubMed: 38103391

Applications

WB

Reactivity

Mouse

Ayantika Kundu 1, Pratiti Ghosh 2, Biswadev Bishayi Verapamil and tangeretin enhances the M1 macrophages to M2 type in lipopolysaccharide-treated mice and inhibits the P-glycoprotein expression by downregulating STAT1/STAT3 and upregulating SOCS3 Int Immunopharmacol, (2024)

PubMed: 38678669

Applications

WB

Reactivity

Mouse

Oskoueian, Ehsan et al. Palm kernel cake extract exerts hepatoprotective activity in heat-induced oxidative stress in chicken hepatocytes BMC Complement Altern Med, 14, 368 (2014)

PubMed: 25273634

Applications

WB

Reactivity

Plant

Tassan Mazzocco, Margherita et al. Exploring the neuroprotective effects of montelukast on brain inflammation and metabolism in a rat model of quinolinic acid-induced striatal neurotoxicity J Neuroinflammation, 20, 34 (2023)

PubMed: 36782185

Applications

IF

Reactivity

Rat

Western blot analysis of rat colon (lane 1), mouse brain (lane 2), mouse heart (lane 3), mouse lung (lane 4), rat ovary (lane 5), mouse testis (lane 6) using iNOS antibody (1 ug/ml)

IHC-P staining of rat brain tissue using anti- iNOS (dilution at 2.5 ug/ml)

Immunohistochemical staining of guinea pig lung tissue using iNOS antibody (dilution of primary antibody - 2.5 ug/ml)

Immunohistochemical staining of paraffin embedded guinea pig heart tissue using anti- iNOS (primary antibody at 2.5 ug/ml)