iNOS antibody

SKU: orb13614

Description

iNOS antibody

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution range: IHC-P: 1:100-600, WB: 1:100-1500

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human iNOS. Please contact us for the exact immunogen sequence. The peptide is available as orb374865.
• Target: iNOS
• Molecular Weight: 131 kDa
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Disclaimer: For research use only

Alternative Names

anti HEP NOS antibody, anti HEP-NOS antibody, anti Hepatocyte NOS antibody, anti HEPNOS antibody, anti Inducible NO synthase antibody, anti Inducible NOS antibody, anti INOS antibody, anti Inosl antibody, anti MAC NOS antibody, anti Nitric oxide synthase 2 inducible antibody, anti Nitric oxide synthase 2A (inducible hepatocytes) antibody, anti Nitric oxide synthase antibody, anti Nitric oxide synthase inducible antibody, anti NOS 2 antibody, anti NOS 2A antibody, anti NOS antibody, anti Nos II antibody, anti NOS type II antibody, anti Nos2 antibody, anti NOS2A antibody

Western blot analysis of rat colon (lane 1), mouse brain (lane 2), mouse heart (lane 3), mouse lung (lane 4), rat ovary (lane 5), mouse testis (lane 6) using iNOS antibody (1 ug/ml)

IHC-P staining of rat brain tissue using anti- iNOS (dilution at 2.5 ug/ml)

Immunohistochemical staining of guinea pig lung tissue using iNOS antibody (dilution of primary antibody - 2.5 ug/ml)

Immunohistochemical staining of paraffin embedded guinea pig heart tissue using anti- iNOS (primary antibody at 2.5 ug/ml)

Quick Database Links

Gene Symbol

iNOS

UniProt

P35228, P29477, Q06518

NCBI Gene ID

4843

RefSeq:000625, 41, 31, 000616

References

Ayantika Kundu,Pratiti Ghosh, Biswadev Bishayi Vitexin along with verapamil downregulates efflux pump P-glycoprotein in macrophages and potentiate M1 to M2 switching via TLR4-NF-κB-TNFR2 pathway in lipopolysaccharide treated mice Immunobiology, 229(1), 152767 (2024)

PubMed: 38103391

Applications

WB

Reactivity

Mouse

Ayantika Kundu 1, Pratiti Ghosh 2, Biswadev Bishayi Verapamil and tangeretin enhances the M1 macrophages to M2 type in lipopolysaccharide-treated mice and inhibits the P-glycoprotein expression by downregulating STAT1/STAT3 and upregulating SOCS3 Int Immunopharmacol, (2024)

PubMed: 38678669

Applications

WB

Reactivity

Mouse

Oskoueian, Ehsan et al. Palm kernel cake extract exerts hepatoprotective activity in heat-induced oxidative stress in chicken hepatocytes BMC Complement Altern Med, 14, 368 (2014)

PubMed: 25273634

Applications

WB

Reactivity

Plant

Tassan Mazzocco, Margherita et al. Exploring the neuroprotective effects of montelukast on brain inflammation and metabolism in a rat model of quinolinic acid-induced striatal neurotoxicity J Neuroinflammation, 20, 34 (2023)

PubMed: 36782185

Applications

IF

Reactivity

Rat