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NOS2A Antibody (Center)

Catalog Number: orb1937965

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb1937965
CategoryAntibodies
DescriptionAffinity Purified Rabbit Polyclonal Antibody (Pab)
Species/HostRabbit
ClonalityPolyclonal
Clone NumberRB18210
Tested applicationsFC, IF, IHC-P, WB
ReactivityHuman
IsotypeRabbit IgG
Antibody TypePrimary Antibody
Dilution rangeIF: 1:25, WB: 1:2000, WB: 1:2000, WB: 1:1000, IHC-P: 1:25, IHC-P: 1:50~100, FC: 1:25
Form/AppearancePurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
ConjugationUnconjugated
MW131117 Da
TargetThis NOS2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 830-860 amino acids from the Central region of human NOS2A.
UniProt IDP35228
NCBINP_000616.3
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Alternative namesNitric oxide synthase, inducible, Hepatocyte NOS,
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NoteFor research use only
Expiration Date12 months from date of receipt.
NOS2A Antibody (Center)

Anti-NOS2A Antibody (Center) at 1:2000 dilution + A549 whole cell lysates.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 131 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

NOS2A Antibody (Center)

Anti-NOS2A Antibody (Center) at 1:2000 dilution + Y79 whole cell lysates.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 131 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

NOS2A Antibody (Center)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung adenocarcinoma epithelial cell line) cells labeling NOS2A at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on A549 cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).

NOS2A Antibody (Center)

NOS2A Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of NOS2A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

NOS2A Antibody (Center)

NOS2A Antibody (Center) western blot analysis in CEM cell line lysates (35 ug/lane). This demonstrates the NOS2A antibody detected the NOS2A protein (arrow).

NOS2A Antibody (Center)

Staining NOS2A in Human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary Antibody.

NOS2A Antibody (Center)

Overlay histogram showing Jurkat cells (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

  • NOS2A Antibody (Center) [orb1168031]

    FC,  IF,  IHC-P,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 30 μl