IL2RA/CD25 Antibody

SKU: orb1538961

Description

IL2RA/CD25 Antibody

Images & Validation

• Tested Applications: ELISA, IHC, IHC-Fr, IHC-P, WB
• Dilution Range: ELISA (4 - 8 µg/ml), IHC, IHC-Fr (5 µg/ml), IHC-P (10 µg/ml), WB (0.2 - 0.4 µg/ml)
• Reactivity: Human
• Application Notes:
  Resuspension: Centrifuge vial prior to opening. Reconstitiute in sterile water to 1.0 mg/ml.. Further information: This in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 10 ug/ml is recommended. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 4-8 ug/ml of this antibody will detect at least 100 pg/ml of recombinant Human sIL-2Ralpha when used in conjunction with compatible secondary reagents. Western Blot: To detect Human sIL-2Ralpha by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human sIL-2Ralpha is 2-4 ng/lane, under non-reducing conditions.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Immunogen: Insect cell derived recombinant Human sIL-2Ralpha.
• Target: IL2RA / CD25
• Purification: Protein G purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Lyophilized from Sterile Solution PBS
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IL2RA, CD25 antigen, CD25, IDDM10, IL-2 receptor subunit alpha, IL2R, Interleukin 2 receptor, alpha, IL-2-RA, IL2-RA, TCGFR, IL-2R subunit alpha, p55, TAC antigen

Quick Database Links

Gene Symbol

IL2RA / CD25