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| Catalog Number | orb461545 |
|---|---|
| Category | Antibodies |
| Description | IL2RA/CD25 Rabbit Polyclonal Antibody (FITC) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | FC |
| Predicted Reactivity | Rat |
| Reactivity | Human, Mouse |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from mouse CD25 (201-268/268aa) |
| Antibody Type | Primary Antibody |
| Concentration | 1mg/ml |
| Dilution range | Flow-Cyt=0.2ug /test |
| Conjugation | FITC |
| Target | IL2RA |
| Storage | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Alternative names | Interleukin 2 receptor alpha chain; CD 25; CD25 an Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Rochana Pramanik 1, Sreya Chattopadhyay 1, Biswadev Bishayi Dual neutralization of TGF-β and IL-21 regulates Th17/Treg balance by suppressing inflammatory signalling in the splenic lymphocytes of Staphylococcus aureus infection-induced septic arthritic mice Immunol Res, (2025)
Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-CD25 antibody (orb461545), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
