IL2RA/CD25 Mouse Monoclonal Antibody

SKU: orb1974720

Description

IL2RA/CD25 Mouse Monoclonal Antibody

Images & Validation

• Tested Applications: FC
• Dilution range: Flow-Cyt=1ug/Test
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a, κ
• Clone No.: 2B2
• Immunogen: KLH conjugated synthetic peptide derived from human IL2RA/CD25
• Target: IL2RA
• Molecular Weight: 28 kDa
• Purification: Affinity purified by Protein G

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Interleukin 2 receptor alpha chain; CD 25; CD25; CD25 antigen; IDDM10; IL 2 RA; IL 2 receptor alpha subunit; IL2 RA; IL-2RA; IL2R; IL2RA; IL2RAC; Interleukin 2 Receptor alpha; Interleukin 2 receptor alpha chain; Interleukin 2 receptor; Ly43; p55; T Cell Growth Factor Receptor; TAC Antigen; TCGFR; IL2RA_HUMAN.

Blank control: SR. Primary Antibody (green line): Mouse Anti-CD25 antibody (orb1974720), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-mouse IgG-FITC, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Mouse IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

scatter diagram showing peripheral blood lymphocytes stained with CD25. The cells were incubated with the antibody (orb1974720) for 30 min at 22°C. The secondary antibody used for 40 min at room temperature. Acquisition of > 10000 events was performed.

Quick Database Links

Gene Symbol

IL2RA