IL12B Antibody

• Catalog Number: orb1270811
• Category: Antibodies
• Description: IL12B Antibody
• Target: IL12B
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Immunogen: This IL12B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264-290 amino acids from the C-terminal region of human IL12B.
• UniProt ID: P29460
• MW: 37 kDa
• Tested applications: FC, IHC-P, WB
• Application notes: For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50For WB starting dilution is: 1:1000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Interleukin-12 subunit beta, IL-12B, Cytotoxic lym
• Research Area: Cancer Biology, Immunology & Inflammation, Signal
• Note: For research use only
• NCBI: P29460

IL12B antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in CEM, MDA-MB453 cell line lysates (35 ug/lane).